Biotechnology: Principles and Processes Questions — NEET Biology

Q1

Agarose extracted from sea weeds finds use in:

A Spectrophotometry

B Tissue culture

C PCR

D Gel electrophoresis

Correct Answer

Option D

Solution

(d) In gel electrophoresis agarose extracted from sea weed is used as gel.

Q2

What is true about plasmid?

A Plasmids are widely used in gene transfer

B These are found in viruses

C Plasmid contains genes for vital activities

D These are main parts of chromosome

Correct Answer

Option A

Solution

(a) Plasmids are small extranuclear circular DNAs widely used in gene transfer.

Q3

Bt cotton variety that was developed by the introduction of toxin gene of Bacillus thuringiensis (Bt) is resistant to

A Fungal diseases

B Plant nematodes

C Insect predators

D Insect pests

Correct Answer

Option D

Solution

(d) Bt cotton is resistant to cotton bollworm (Insect pest).

Q4

Organelle/organoid involved in genetic engineering is

A plasmid

B mitochondrion

C golgi apparatus

D lomasome

Correct Answer

Option A

Solution

(a) Plasmid is used as vector in genetic engineering and can replicate independently.

Q5

The plasmid

A helps in respiration

B genes found inside nucleus

C is a component of cell wall of bacteria

D is the genetic part in addition to DNA in micro-organisms

Correct Answer

Option D

Solution

(d) Plasmid is a small circular molecule of DNA found in bacterial cell in addition to the larger circular bacterial DNA.

Q6

In bacterial chromosomes, the nucleic acid polymers are

A linear DNA molecule

B circular DNA molecule

C of two types-DNA and RNA

D linear RNA molecule

Correct Answer

Option B

Solution

(b) Bacterial chromosomes comprise of circular DNA.

Q7

The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification.

A B - Denaturation at a temperature of about 98C separating the two DNA strands.

B A - Denaturation at a temperature of about 50C.

C C - Extension in the presence of heat stable DNA polymerase.

D A - Annealing with two sets of primers.

Correct Answer

Option C

Solution

(c) C - Extension or elongation of the strands using heat-stable DNA polymerases at 72C.

Q8

In genetic engineering, the antibiotics are used

A as selectable markers.

B to select healthy vectors.

C to keep the cultures free of infection.

D as sequences from where replication starts.

Correct Answer

Option A

Solution

(a) In genetic engineering, the antibiotics are used as selectable markers.

Q9

Biolistics (gene-gun) is suitable for

A DNA fingerprinting.

B Disarming pathogen vectors.

C Transformation of plant cells.

D Constructing recombinant DNA by joining DNA fragments with vectors.

Correct Answer

Option C

Solution

(c) Biolistic gene gun is used for transformation of plant cells.

Q10

For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of :

A Silver or Platinum

B Platinum or Zinc

C Silicon or Platinum

D Gold or Tungsten

Correct Answer

Option D

Solution

(d) Microparticles made of tungsten and gold coated with foreign DNA are bombarded into target cells in gene gun.