Biotechnology: Principles and Processes

NEET Biology · 64 questions · Page 2 of 7 · Click an option or "Show Solution" to reveal answer

Q11

Which one is a true statement regarding DNA polymerase used in PCR

A It is used to ligate introduced DNA in recipient cell

B It serves as a selectable marker

C It is isolated from a virus

D It remains active at high temperature

Correct Answer

Option D

Solution

(d) Taq DNA polymerase is a thermostable enzyme which remains active at high temperature.

Q12

PCR and Restriction Fragment Length Polymorphism are the methods for :

A Study of enzymes

B Genetic transformation

C DNA sequencing

D Genetic Fingerprinting

Correct Answer

Option D

Solution

(d) PCR and RFLP are the methods used for genetic fingerprinting.

Q13

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by :

A Polymerase chain reaction

B Electrophoresis

C Restriction mapping

D Centrifugation

Correct Answer

Option B

Solution

(b) DNA fragments generated by restriction endonucleases can be separated by gel electrophoresis.

Q14

Commonly used vectors for human genome sequencing are:

A T-DNA

B BAC and YAC

C Expression Vectors

D kT / A Cloning Vectors

Correct Answer

Option B

Solution

(b) Commonly used vectors for human genome sequencing are BAC (Bacterial artificial chromosome) and YAC (Yeast artificial chromosome).

Q15

In vitro clonal propagation in plants is characterized by:

A PCR and RAPD

B Northern blotting

C Electrophoresis and HPLC

D Microscopy

Correct Answer

Option A

Solution

(a) PCR and RAPD techniques are used for the characterisation of in vitro clonal propagation in plants.

Q16

The process of separation and purification of expressed protein before marketing is called:

A Downstream processing

B Bioprocessing

C Postproduction processing

D Upstream processing

Correct Answer

Option A

Solution

(a) Downstream processing includes separation and purification of product after fermentation.

Q17

Choose the correct pair from the following

A Polymerases - Synthesize DNA strands

B Nucleases - Cut the two strands of DNA

C Exonucleases - Make cuts at the ends of DNA strands

D Ligases - Join the two DNA molecules

Correct Answer

Option A

Solution

(a) Ligases join two DNA molecules. Polymerases synthesize DNA strands.

Q18

Match the following enzymes with their functions: Column-I Column-II

A -(iv),

B -(iii),

C -(i),

D -(ii)

Correct Answer

Option C

Solution

DNA ligase joins DNA strands.

Taq polymerase copies DNA at high temperature.

Q19

A selectable marker is used to :

A Mark a gene on a chromosome for isolation using restriction enzyme.

B Help in eliminating the non-transformants, so that the transformants can be regenerated.

C Identify the gene for a desired trait in an alien organism.

D Select a suitable vector for transformation in a specific crop.

Correct Answer

Option B

Solution

(b) Selectable marker helps in identification and elimination of non-transformants whilst permitting selective growth of transformants.

Q20

The sequence that controls the copy number of the linked DNA in the vector, is termed

A Ori site

B Palindromic sequence

C Recognition site

D Selectable marker

Correct Answer

Option A

Solution

(a) Ori site controls the copy number of the linked DNA in the vector.

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