Biotechnology: Principles and Processes — Page 5 | NEET Biology

Q41

Genes of interest can be selected from a genomic library by using

A Restriction enzymes

B Cloning vectors

C DNA probes

D Gene targets

Correct Answer

Option C

Solution

(c) DNA probes are used to detect the presence of complementary sequences.

Q42

The colonies of recombinant bacteria appear white in contrast to blue colonies of nonrecombinant bacteria because of:

A Insertional inactivation of alphagalactosidase in non-recombinant bacteria

B Insertional inactivation of alphagalactosidase in recombinant bacteria

C Inactivation of glycosidase enzyme in recombinant bacteria

D Non-recombinant bacteria containing beta-galactosidase

Correct Answer

Option D

Solution

(d) Insertional inactivation of beta-galactosidase in recombinant bacteria causes white colonies.

Q43

During the process of isolation of DNA, chilled ethanol is added to

A Remove proteins such as histones

B Precipitate DNA

C Break open the cell to release DNA

D Facilitate action of restriction enzymes

Correct Answer

Option B

Solution

(b) DNA is insoluble in ethanol and precipitates out of solution.

Q44

Which vector can clone only a small fragment of DNA?

A Bacterial artificial chromosome

B Yeast artificial chromosome

C Plasmid

D Cosmid

Correct Answer

Option C

Solution

(c) Plasmids are small extranuclear circular DNAs used as vectors for small DNA fragments.

Q45

An analysis of chromosomal DNA using the Southern hybridization technique does not use:

A Electrophoresis

B Blotting

C Autoradiography

D PCR

Correct Answer

Option D

Solution

(d) PCR is not used in Southern hybridization technique.

Q46

The cutting of DNA at specific locations became possible with the discovery of

A Probes

B Selectable markers

C Ligases

D Restriction enzymes

Correct Answer

Option D

Solution

(d) Restriction enzymes are used to cut DNA at specific locations.

Q47

Which of the following is a restriction endonuclease?

A Hind II

B Protease

C DNase I

D RNase

Correct Answer

Option A

Solution

(a) Hind II is a type of restriction endonuclease.

Q48

The taq polymerase enzyme is obtained from

A Thermus aquaticus

B Thiobacillus ferroxidans

C Bacillus subtilis

D Pseudomonas putida

Correct Answer

Option A

Solution

(a) The Taq polymerase enzyme is obtained from Thermus aquaticus which lives in hot springs.

Q49

Which of the following is not a feature of the plasmids?

A Independent replication

B Circular structure

C Transferable

D Single - stranded

Correct Answer

Option D

Solution

(d) Plasmid is an extra chromosomal, double stranded circular DNA.

Q50

What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?

A The smaller the fragment size, the farther it moves

B Positively charged fragments move to farther end

C Negatively charged fragments do not move

D The larger the fragment size, the farther it moves

Correct Answer

Option A

Solution

(a) DNA fragments separate according to their size through sieving effect provided by agarose gel.