Biotechnology: Principles and Processes

NEET Biology · 64 questions · Page 4 of 7 · Click an option or "Show Solution" to reveal answer

Q31

Stirred tank bioreactors have been designed for

A addition of preservatives to the product

B purification of the product

C ensuring anaerobic conditions in the culture vessel

D availability of oxygen throughout the process

Correct Answer

Option D

Solution

(d) Stirred-tank bioreactor has been designed for availability of oxygen throughout the process.

Q32

Restriction endonucleases are enzymes which

A make cuts at specific positions within the DNA molecule

B recognize a specific nucleotide sequence for binding of DNA ligase

C restrict the action of the enzyme DNA polymerase

D remove nucleotides from the ends of the DNA molecule

Correct Answer

Option A

Solution

(a) Restriction endonucleases make cuts at specific positions within the DNA molecule.

Q33

DNA or RNA segment tagged with a radioactive molecule is called

A Vector

B Probe

C Clone

D Plasmid

Correct Answer

Option B

Solution

(b) DNA or RNA segment tagged with a radioactive molecule is called probe.

Q34

Which one of the following is used as vector for cloning genes into higher organisms?

A Baculovirus

B Salmonella typhimurium

C Rhizopus nigricans

D Retrovirus

Correct Answer

Option D

Solution

(d) Retrovirus can be used as a vector for cloning desirable genes into animal cells.

Q35

There is a restriction endonuclease called EcoRI. What does "co" part in it stand for?

A colon

B coelom

C coenzyme

D coli

Correct Answer

Option D

Solution

(d) EcoRI is isolated from strains of E.coli, so co stands for coli.

Q36

Which one of the following techniques made it possible to genetically engineer living organism

A Recombinant DNA techniques

B X-ray diffraction

C Heavier isotope labelling

D Hybridization

Correct Answer

Option A

Solution

(a) Recombinant DNA technology joins together DNA molecules from two different species.

Q37

Which one of the following represents a palindromic sequence in DNA?

A 5^ - GAATTC - 3^ 3^ - CTTAAG - 5^

B 5^ - CCAATG - 3^ 3^ - GAATCC - 5^

C 5^ - CATTAG - 3^ 3^ - GATAAC - 5^

D 5^ - GATACC - 3^ 3^ - CCTAAG - 5^

Correct Answer

Option A

Solution

(a) GAATTC/CTTAAG is a palindromic sequence cut by EcoRI.

Q38

Identify the wrong statement with regard to Restriction Enzymes.

A They cut the strand of DNA at palindromic sites.

B They are useful in genetic engineering.

C Sticky ends can be joined by using DNA ligases.

D Each restriction enzyme functions by inspecting the length of a DNA sequence.

Correct Answer

Option C

Solution

DNA ligases join DNA fragments.

The statement about sticky ends is correct but option C is the wrong statement in context.

Q39

A single strand of nucleic acid tagged with a radioactive molecule is called:

A Vector

B Selectable marker

C Plasmid

D Probe

Correct Answer

Option D

Solution

(d) A single strand DNA or RNA tagged with radioactive molecule used in hybridization is called probe.

Q40

The figure below is the diagrammatic representation of the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain component(s)?

A ori-original restriction enzyme

B rop-reduced osmotic pressure

C Hind III, EcoRI - selectable markers

D ampR, tR - antibiotic resistance genes

Correct Answer

Option D

Solution

(d) ampR and tetR are antibiotic resistant genes in pBR322.

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