Biotechnology: Principles and Processes

NEET Biology · 64 questions · Page 3 of 7 · Click an option or "Show Solution" to reveal answer

Q21
The plasmids present in the bacterial cells are
A circular double helical DNA molecules
B circular double helical RNA molecules
C linear double helical DNA molecules
D linear double helical RNA molecules.
Correct Answer
Option A
Solution

(a) Plasmids are the extrachromosomal double stranded DNA.

Q22
Two bacteria found to be very useful in genetic engineering experiments are
A Escherichia and Agrobacterium
B Nitrobacter and Azotobacter
C Rhizobium and Diplococcus
D Nitrosomonas and Klebsiella
Correct Answer
Option A
Solution

(a) Escherichia and Agrobacterium are both used as vectors in genetic engineering.

Q23
Introduction of food plants developed by genetic engineering is not necessarily undesirable because
A economy of developing countries may suffer
B these products are less tasty than the existing products
C this method is costly
D there is danger of entry of viruses and toxins with introduced crops
Correct Answer
Option D
Solution

(d) By consuming transgenic plants there is a danger of entry of viruses and toxins causing different types of allergies and other health hazards to human beings.

Q24
In bacteria, plasmid is
A extra-chromosomal material
B main DNA
C non-functional DNA
D repetitive gene
Correct Answer
Option A
Solution

(a) A plasmid is an independent, circular, self-replicating DNA molecule that carries only a few genes.

Q25
Chromosomes in a bacterial cell can be 1 in number and
A can be circular as well as linear within the same cell
B are always round
C are not always linear
D can be either circular or linear, but never both within the same cell
Correct Answer
Option B
Solution

(b) Prokaryotes (bacteria) have circular chromosomes.

Q26
What is antisense technology?
A A cell displaying a foreign antigen used for synthesis of antigens
B Production of somaclonal variants in tissue cultures
C When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene
D RNA polymerase producing DNA
Correct Answer
Option C
Solution

(c) Antisense RNA undergoes base pairing with its complementary mRNA sequence blocking gene expression.

Q27
The specific palindromic sequence which is recognized by EcoRl is
A 5^ - GGAACC - 3^ 3^ - CCTTGG - 5^
B 5^ - CTTAAG - 3^ 3^ - GAATTC - 5^
C 5^ - GGATCC - 3^ 3^ - CCTAGG - 5^
D 5^ - GAATTC - 3^ 3^ - CTTAAG - 5^
Correct Answer
Option D
Solution

(d) The specific palindromic sequence recognized by EcoRI is 5-GAATTC-3 / 3-CTTAAG-5.

Q28
The linking of antibiotic resistance gene with the plasmid vector became possible with
A DNA ligase
B Endonucleases
C DNA polymerase
D Exonucleases
Correct Answer
Option A
Solution

(a) DNA ligase joins the antibiotic resistance gene with the plasmid vector.

Q29
Which one of the following is commonly used in transfer of foreign DNA into crop plants?
A Meloidogyne incognita
B Agrobacterium tumefaciens
C Penicillium expansum
D Trichoderma harzianum
Correct Answer
Option B
Solution

(b) Agrobacterium tumefaciens Ti plasmid is widely used in plant engineering as a vector.

Q30
Polyethylene glycol method is used for
A biodiesel production
B seedless fruit production
C energy production from sewage
D gene transfer without a vector
Correct Answer
Option D
Solution

(d) Polyethylene glycol mediated DNA uptake is a direct gene transfer method.

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